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2013 ASHS Annual Conference

14496:
Mitigation of Salinity Induced Morpho-physiological and Biochemical Effects by Seed Treatment with 28-Homobrassinolide in Pea (Pisum sativum L.)

Tuesday, July 23, 2013: 5:30 PM
Springs Salon A/B (Desert Springs J.W Marriott Resort )
Muhammad Adnan Shahid, University College of Agriculture, University of Sargodha, Sargodha, Pakistan
Rashad M. Balal, Department of Horticulture, Department of Horticulture, Faculty of Agriculture, University of Sargodha, Sargodha, Pakistan
Muhammad Aslam Pervez, University of Agriculture, Faisalabad, Pakistan
Zafar Iqbal, University College of Agriculture, University College of Agriculture, University of Sargodha, Sargodha, Pakistan
Neil Mattson, Department of Horticulture, Cornell University, Ithaca, NY
Francisco Garcia-Sanchez, Dpto. Nutrición Vegetal., University of Murcia, Murcia, Spain
Salinity is very severe global problem so a study was carried out to investigate the response of pea (Pisum sativum L.) cv. Climax seeds imbibed with 28-homobrassinolide (HBL) and sodium chloride (NaCl) before sowing. The seeds soaked in two different concentrations of HBL (5 and 10 µM) for 4 hours, caused an increase in most of the aspects of shoot and root growth both at seedling stage and mature stage (90 days after sowing). At seedling stage, HBL (10 µM ) significantly enhanced the fresh biomass by 19%, dry biomass by 31%, seedling height (shoot+root) by 14%, photosynthesis rate by 29%, stomatal conductance by 18%, total chlorophyll contents by 43%, superoxide dismutase (SOD) by 39%, nitrare reductase activity (NRA) by 28% and nitrite reductase activity (NiRA) by 29% in comparison to control (water soaked alone). However, at mature plant stage the plants grown from seeds pre-imbibed in HBL (10µM) possessed 14% and 27% higher leaf NR and NiR activities, 23% increase in dry mass, 51% enhancement in nodule number, and 20% increase in nodule dry biomass, in comparison to water imbibed, control. Antioxidant enzyme superoxide SOD was 66% higher than control. The 28-homobrassinolide enhanced the photosynthesis rate (28%), stomatal conductance (31%), chlorophyll a (48%) and chlorophyll b (59%) as compared to the control. It was concluded that salinity induced drastic effects can be mitigated by the seed treatment with 28-homobrassinolide.
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